Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
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چکیده
منابع مشابه
Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio frequency alternating magnetic field is applied, ferromagnetic particles dissipate heat and DNA ligas...
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ژورنال
عنوان ژورنال: Biochemistry and Biophysics Reports
سال: 2016
ISSN: 2405-5808
DOI: 10.1016/j.bbrep.2016.10.006